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Heifers may not be capable of successful conception until after several oestrus cycles. This ensures maximal damage and means that bullets are less likely to exit the skull. As few as 12 million sperm per insemination have given good conception rates. Dihydro-streptomycin Another method which gained popularity was the "speculum" method. Metricheck Metricheck, Simcro, New Zealand , a device consisting of a stainless steel rod with a rubber hemisphere, can also be used to retrieve vaginal contents. Urine is best sampled mid stream, cows can be stimulated to urinate by gentle stimulation of the perineum.
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Different A.I. techniques and method for fertility

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The Healthy Cow

Frontal or poll shots can be used. Improper cleaning of instruments and in sanitary conditions may lead to lower fertility. The target is the intersection of line between inside the corner of eye and base of the horn or where the horn would be in polled animals which is slightly above the ear. Also, male animals are often more strong, powerful, and potentially ill-mannered and thus require special housing and handling equipment. It is associated with the presence of pyrexia, fetid pus within the uterine lumen, vagina and discharging from the vulva accompanied with delayed uterine involution. The muzzle of the captive bolt must be pressed against the head of the animal.
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Woman on top - Wikipedia

It has been most widely used for breeding dairy cattle and has made bulls of high genetic merit available to all. This will vary with the cow's diet but extremely firm faeces can be associated with metabolic problems and very liquid faeces can be associated with intestinal disease, particularly if it smells foul or contains blood. In , Ivanoff of Russia pioneered AI research in birds, horses, cattle and sheep. Much research work has been conducted on this subject. In addition, natural breeding is physically stressful.
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Based on the size and structure of the bovine reproductive tract, two methods are accepted as proper methods for insemination in beef cattle. In animals previously inseminated, the catheter should not be forced through the cervix since pregnancy is a possibility. Alternatively, cycling heifers whose nutrition is severely restricted quickly display anoestrus even though they have exceeded the critical pubertal bodyweight. This may be seen by a cow kicking at her abdomen, repeatedly lying down and standing up or increased vocalisation. Penetrating captive bolt guns are also known as slaughter pistols, stunners and humane killers. Before shooting, look down the line of fire and check where an exiting bullet might travel. Other knives may be used but if the knife does not have a sharp point, it will require cutting from the skin inward.
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